Journal: Journal of Pharmaceutical Analysis

Article Title: Altered synaptic currents, mitophagy, mitochondrial dynamics in Alzheimer's disease models and therapeutic potential of Dengzhan Shengmai capsules intervention

doi: 10.1016/j.jpha.2023.10.006

Figure Lengend Snippet: Dengzhan Shengmai (DZSM) alleviates Aβ 42 -induced mitochondrial dysfunction in primary neural cells. (A) Representative flow cytograms and quantitative analysis of cellular reactive oxygen species (ROS). (B) Representative flow cytograms of cellular mitochondrial membrane potential (MMP) and the quantitative analysis of the ratio of red to green fluorescence. (C) Statistical histogram of cellular ATP content. (D) Representative images of MitoTracker Red CMXRos staining and the quantitative analysis of mitochondrial length (magnification = 400 ×; 80–100 mitochondria were selected for analysis, n = 3). (E) Representative transmission electron microscopy (TEM) images of cells (magnification = 7,000 ×; 20–30 mitochondria were selected for analysis, n = 3), and the quantitative analysis of the length and area of mitochondria. Experimental data are expressed as the mean ± standard error of mean (SEM), n = 3. ∗∗ P < 0.01 vs. Control group, and # P < 0.05 and ## P < 0.01 vs. Aβ 42 group. Mdivi1: mitochondrial division inhibitor 1.

Article Snippet: Trypsin (T1360), CCK8 kit (cat: CA1210), ATP detection kit (cat: BC0300), MMP Assay Kit with JC-1 (M8650), ROS assay kit (cat: CA1410), and BCA protein assay kit (cat: PC0020) were purchased from Solarbio Science & Technology (Beijing, China).

Techniques: Membrane, Fluorescence, Staining, Transmission Assay, Electron Microscopy

Journal: American Journal of Translational Research

Article Title: Knockdown of miR-27a reduces TGFβ-induced EMT and H 2 O 2 -induced oxidative stress through regulating mitochondrial autophagy

doi:

Figure Lengend Snippet: Downregulating miR-27a reduced H2O2-induced oxidative stress. A. ESCs were treated with or without H2O2. The miR-27a RNA level was examined by qPCR. ESCs, transfected with or without inhibitor-NC and inhibitor-27, were then intervened by 100 μM H2O2. B. Intracellular ROS production was detected with DCFH-DA assay. C. Western blotting was conducted to examine Bcl-2 and Bax protein levels. D. Flow cytometry was carried out for cell apoptosis examination. E. Total p65 and phosphorylated p65 levels was accessed by western blotting. Data were presented as mean ± SD. **P<0.01, ***P<0.001.

Article Snippet: The ROS Assay Kit (Solarbio, China) was utilized to determine intracellular ROS levels.

Techniques: Transfection, DCFH-DA Assay, Western Blot, Flow Cytometry

Journal: American Journal of Translational Research

Article Title: Knockdown of miR-27a reduces TGFβ-induced EMT and H 2 O 2 -induced oxidative stress through regulating mitochondrial autophagy

doi:

Figure Lengend Snippet: miR-27a regulates mitophagy in H2O2-treated ESCs. A. Mimic-NC, mimic-27, inhibitor-NC, and inhibitor-27 were transfected into ESCs, respectively. PINK1, Parkin, and LC3 protein levels were examined by western blotting. ESCs, transfected with inhibitor-NC, inhibitor-27, and inhibitor-27+si-PINK1, were then intervened by 100 μM H2O2. B. PINK1, Parkin, and LC3 protein levels were examined by western blotting. C. Intracellular ROS production was detected by DCFH-DA assay. D. Western blotting was carried out to determine Bcl-2 and Bax protein levels. E. Cell apoptosis was examined by flow cytometry. Data were presented as mean ± SD. *, **, and *** represent P<0.05, P<0.01, and P<0.001, respectively.

Article Snippet: The ROS Assay Kit (Solarbio, China) was utilized to determine intracellular ROS levels.

Techniques: Transfection, Western Blot, DCFH-DA Assay, Flow Cytometry